Copyright@2011 University of South Carolina
Wang Research Group
                          Keck Foundation Grant Funds Open Lab


With an $800,000 grant from the W.M. Keck Foundation, a leading supporter of high-impact medical research, science, and engineering, USC is creating an open laboratory in its NanoCenter to develop new technologies in tissue engineering, sensing, drug delivery, vaccine manufacturing, and other biomedical applications.

The W.M. Keck Open Laboratory for Bionanoparticle Technology Discovery and Development is a partnership between USC and the Scripps Research Institute in La Jolla, Calif. Two faculty members in USC s Department of Chemistry and Biochemistry, Qian Wang and Cathy Murphy, have teamed with Scripps chemistry professor M.G. Finn to pursue this cutting-edge research.

Meanwhile, development of the 1,500-square-foot bionanoparticle laboratory has begun in the USC NanoCenter in Sumwalt College. The facility will include separate rooms for ultracentrifuges, incubators, liquid chromatography systems, and other specialized equipment such as a state-of-the-art ICP-OES, DSC, and NIR fluoriphotometers.

The W.M. Keck Open Laboratory will provide exciting educational opportunities for high school, undergraduate, and graduate students. Some will be trained to use the lab s high-tech instruments, and many more will be exposed to the possibilities of bionanoparticle research.
Team Members

Qian Wang
Professor, Department of Chemistry and Biochemistry and Nanocenter, University of South Carolina
Tel: 803-777-8436
Fax: 803-777-9521
E-mail: wang@mail.chem.sc.edu

M. G. Finn
Professor, Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037
Tel: 858-784-8845
Fax: 858-784-8850
E-mail: mgfinn@scripps.edu
Facilities

Keck Foundation Grant Funds Open Lab is operated on a costless basis for all UCS nanocenter members. However,  fees are accessed for non-nanocenter members for consumables  and  equipment use. The major shared instruments in the Keck Lab  include:

Atomic Force Microscopy (AFM)

Florescence Microscopy

Inductively Coupled Plasma Mass Spectroscopy (ICP-MS)

Cell Culture Incubator

Real Time PCR


Use Policies


It is imperative that all users work responsibly in the lab.

Access: The Keck Lab is open Monday ~ Friday from 8:30 AM to 5:30 PM. It is closed on all major holidays. Most of the major equipment is available for use after hours.

Scheduling: The major instruments are signed-up on a first come first serve basis. Any interested user can also call (777-2680) to reserve instruments or simply walk in to the Keck Lab to sign up ahead of time.

The following rules and regulations must be followed by individuals who intend to work in this lab.

1. All new users must go through instrument training.

2. Any instrument use must be entered in the appropriate log books.

3. If in doubt about using any equipment or chemical please ask the Keck Lab staff. Any mistake on your part could put you and others at great risk. Proper chemical handling is expected from all users.

4. Closed toed foot wear is mandatory for all lab users.

5. In case of a spill it is very important that you immediately inform the Keck Lab staff. The staff will help you clean up the spill in an efficient and safe way.

6. Users are requested to keep their appointments and clean up after they use this facility.
Publications

Since January 2006, the PIs have published or submitted 18 related papers, all with the theme of bionanoparticle or inorganic nanoparticle fabrication and assembly, which acknowledged the support of the W. M. Keck Foundation.

   Please email us for reprints or PDF files
1. Duane E. Prasuhn Jr., Pratik Singh, Erica Strable, Steven Brown, Marianne Manchester, M.G. Finn, "Plasma Clearance of Bacteriophage Qb Particles as a Function of Surface Charge", J. Am. Chem. Soc.  2007, in press.

2. Kaltgrad, E.; Sen Gupta, S.; Punna, S.; Huang, C.-Y.; Chang, A.; Wong, C.-H. Finn, M.G.; Blixt, O. "Anti-Carbohydrate Antibodies Elicited by Polyvalent Display on a Viral Scaffold", ChemBioChem 2007, 8, 1455-1462.

3. Catherine J. Murphy, Anand M. Gole, Simona E. Hunyadi, John W. Stone, Patrick N. Sisco, Alaaldin Alkilany, Brian E. Kinard, Patrick Hankins, "Chemical Sensing and Imaging with Metallic Nanorods",  Chem. Comm., 2007, in press.

4. Anand Gole, Catherine J. Murphy,"Azide-Derivatized Gold Nanorods: Functional Materials for "Click" Chemistry", Langmuir, in press.

5. Zhao, Y. J.;  Li, S.; Davidson, A.;  Yang, B.; Bain, J.; Wang, Q.; Lin, Q.; "A MEMS Viscometric Device for Continuous Glucose Monitoring", J. Micromechanics and Microengineering, accepted.

6. Bruckman, M.; Kaur, G.; Lee; L. A.; Xie, F.; Sepulveda, J.; Breitenkamp, R.; Zhang, X.; Joralemon, M.; Russell, T. P.; Emrick, T.; Wang, Q.; "Surface modification of tobacco mosaic virus with click chemistry and cell adhesion properties", Chembiochem, accepted.

7. Lin, Y.; Su, Z.; Niu, Z.; Li, S.; Kaur, G.; Lee, L. A.; Wang, Q.; "Layer-by-Layer Assembly of Viral Capsid for Cellular Adhesion", Acta Biomater., accepted.

8. Niu, Z.; Li, S.; Bruckman, M.; Lee, L. A.; Lee, B.; Pingali, S. V.; Thiyagarajan, P.; Wang, Q.; "Synthesis and Characterization of Tobacco Mosaic Virus Templated Polymeric Nanomaterials", ACS Symposium Series, Invited.

9. Lee, B.; Lo, C.-T.; Thiyagarajan, P.; Winans, R. E.; Li, X.; Niu, Z.; Wang, Q.; "Effect of Interfacial Interaction on the Cross-Sectional Morphology of Tobacco Mosaic Virus Using GISAXS", Langmuir,  2007, in press.

10. Wang, X.; Niu, Z.; Li, S.; Wang, Q.; Li, X.; "Nanomechanical characterization of polyaniline coated tobacco mosaic virus nanotubes", J. Biomedical Mater. Res. Part A. 2007, in press.

11. Niu, Z.; Bruckman, M.; Li, S.; Lee, L. A.; Lee, B.; Pingali, S. V.; Thiyagarajan, P.; Wang, Q.; "Assembly of Tobacco Mosaic Virus into Fibrous and Macroscopic Bundled Arrays by Aniline Polymerization on its Surface", Langmuir, 2007, 23, 6719-6724.

12. Barnhill, H. N.; Claudel-Gillet, S.; Ziessel, R.; Charbonni¨¨re, L.; Wang, Q.; "A Prototype Protein Assembly as Scaffold for Time-Resolved Fluoroimmuno Assays", J. Am. Chem. Soc. 2007, 129, 7799-7806.

13. Barnhill, H. N.; Reuther R.; Ferguson, P. L.; Dreher, T.; Wang, Q.; "Turnip Yellow Mosaic Virus as A Chemoaddressable Bionanoparticle", Bioconj. Chem.  2007, 18, 852-859.

14. Bruckman, M.; Niu, Z.; Li, S.; Lee, L. A.; Varazo, K.; Nelson, T.; Lavigne, J. J.; Wang, Q.; "Development of Nanobiocomposite Fibers by Controlled-Assembly of Rod-Like Tobacco Mosaic Virus", NanoBiotechnology, 2007, 3, 31-39.

15. Zeng, Q.; Li, T.; Cash, B.; Li, S.; Xie, F.; Wang, Q.; "Chemoselective derivatization of a bionanoparticle by click reaction and ATRP reaction", Chem. Commun. 2007, 1453-1455.

16. Lee, L. A.; Wang, Q.; "Adaptations of Nanoscale Viruses and Other Protein Cages for Medical Applications", Nanomedicine, 2006, 2, 137-149.

17. Lee, L. A.; Barnhill, H. N.; Wang, Q.; "Bionanoparticles and the Biomedicinal Applications", in "Nanomaterials for Medical Diagnosis and Therapy" (Series: Nanotechnologies for Life Sciences, Vol. 9), Ed. Challa S. S. R. Kumar, Wiley-CH, Weinheim, pp 557-596 (2007).

18. Niu, Z.; Bruckman, M.; Kotakadi, V.S.; He, J.; Emrick, T.; Russell, T.P.; Yang, L.; Wang, Q.; "Study and characterization of tobacco mosaic virus head-to-tail assembly assisted by aniline polymerization", Chem. Commun. 2006, 3019-3021.

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